8-well, 1.5 mm-thick cover glass chambers Search Results


sealed polyethylene vials 32 15 thick  (AgriSense BCS Ltd)
90
AgriSense BCS Ltd sealed polyethylene vials 32 15 thick
Sealed Polyethylene Vials 32 15 Thick, supplied by AgriSense BCS Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/sealed+polyethylene+vials+32+15+thick/10__1016_slash_j__cropro__2011__09__008-40-9-16
Average 90 stars, based on 1 article reviews
sealed polyethylene vials 32 15 thick - by Bioz Stars, 2026-10
90/100 stars
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borosilicate glasses corning® eagle xg  (Corning Life Sciences)
90
Corning Life Sciences borosilicate glasses corning® eagle xg
Borosilicate Glasses Corning® Eagle Xg, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/borosilicate+glass+corning+eagle+xg/us10823365-64-30-24
Average 90 stars, based on 1 article reviews
borosilicate glasses corning® eagle xg - by Bioz Stars, 2026-10
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acid washed #1.5 coverslips  (Corning Life Sciences)
90
Corning Life Sciences acid washed #1.5 coverslips
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Acid Washed #1.5 Coverslips, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/acid+washed+glass+coverslips/pmc05764572-269-7-11
Average 90 stars, based on 1 article reviews
acid washed #1.5 coverslips - by Bioz Stars, 2026-10
90/100 stars
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scintillator plates and pmts  (Taito corporation)
90
Taito corporation scintillator plates and pmts
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Scintillator Plates And Pmts, supplied by Taito corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/scintillator+plates+and+pmts/10__1016_slash_j__nima__2012__05__079-101-3-28
Average 90 stars, based on 1 article reviews
scintillator plates and pmts - by Bioz Stars, 2026-10
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li chips thick diameter  (MTI Corporation)
90
MTI Corporation li chips thick diameter
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Li Chips Thick Diameter, supplied by MTI Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/li+chips+thick+diameter/10__1016_slash_j__nanoen__2017__01__042-64-0-11
Average 90 stars, based on 1 article reviews
li chips thick diameter - by Bioz Stars, 2026-10
90/100 stars
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aluminum foil  (MTI Corporation)
90
MTI Corporation aluminum foil
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Aluminum Foil, supplied by MTI Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/aluminum+foil/10__1039_slash_d3ta01180a-68-6-11
Average 90 stars, based on 1 article reviews
aluminum foil - by Bioz Stars, 2026-10
90/100 stars
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borosilicate glasses schott n-sk2  (SCHOTT)
90
SCHOTT borosilicate glasses schott n-sk2
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Borosilicate Glasses Schott N Sk2, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/borosilicate+glass+schott+8250/us10823365-64-17-28
Average 90 stars, based on 1 article reviews
borosilicate glasses schott n-sk2 - by Bioz Stars, 2026-10
90/100 stars
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silicone rubber sheets (1.5 mm thick)  (RS Components)
90
RS Components silicone rubber sheets (1.5 mm thick)
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Silicone Rubber Sheets (1.5 Mm Thick), supplied by RS Components, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/silicone+rubber/pmc11304267-248-7-16
Average 90 stars, based on 1 article reviews
silicone rubber sheets (1.5 mm thick) - by Bioz Stars, 2026-10
90/100 stars
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titanium disks  (Fullerton Technology Co Ltd)
90
Fullerton Technology Co Ltd titanium disks
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Titanium Disks, supplied by Fullerton Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/titanium+disks/pmc05656347-38-0-31
Average 90 stars, based on 1 article reviews
titanium disks - by Bioz Stars, 2026-10
90/100 stars
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ti foils  (Nilaco corp)
90
Nilaco corp ti foils
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Ti Foils, supplied by Nilaco corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/ti+foil/10__1016_slash_j__matlet__2014__09__032-27-1-8
Average 90 stars, based on 1 article reviews
ti foils - by Bioz Stars, 2026-10
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6% polyacrylamide gels (1.5 thick)  (PanReac AppliChem)
90
PanReac AppliChem 6% polyacrylamide gels (1.5 thick)
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
6% Polyacrylamide Gels (1.5 Thick), supplied by PanReac AppliChem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/6++polyacrylamide+gels++1+5+thick+/pm40082964-175-6-17
Average 90 stars, based on 1 article reviews
6% polyacrylamide gels (1.5 thick) - by Bioz Stars, 2026-10
90/100 stars
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sheets acrylic  (Formosa Idemitsu Petrochemical)
90
Formosa Idemitsu Petrochemical sheets acrylic
( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick <t>coverslips</t> for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.
Sheets Acrylic, supplied by Formosa Idemitsu Petrochemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/8-well%2C+1%2E5+mm-thick+cover+glass+chambers/sheets+acrylic/pmc06889311-209-14-20
Average 90 stars, based on 1 article reviews
sheets acrylic - by Bioz Stars, 2026-10
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Image Search Results


( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick coverslips for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.

Journal: eLife

Article Title: An optimized method for 3D fluorescence co-localization applied to human kinetochore protein architecture

doi: 10.7554/eLife.32418

Figure Lengend Snippet: ( A ) Fixation: 3% PFA in PHEM buffer without any detergent was used as standard fixation in this study (Details in Text and Methods). ( B ) Staining: Fixed samples were immunofluorescently labeled with antibodies to enhance S/N. Samples were usually mounted with Prolong Gold. ( C ) Imaging was performed by spinning disk confocal microscopy (See Materials and methods for details). ( D ) For CA correction, Hec1-9G3 monoclonal antibody was labeled by green and red tagged secondary antibodies (Hec1-9G3-GR). ( E ) Measurements: X, Y, Z coordinates of the centroids of green and red kinetochore fluorescence were determined by 3D Gaussian fitting. Details of equations for separation measurements are in Text, Figures, and Methods. ( F ) Error analysis: computer simulations were used for error analysis of the method. The sources of potential errors in separation measurements were highlighted in pink underline with *. 10.7554/eLife.32418.003 Figure 1—source data 1. Pipeline for obtaining mean 3D separation measurements with nm-scale accuracy. Critical factors were: (1) prepare bright high signal to noise samples to reduce standard deviation (SD) of centroid determination; (2) use mounting media like ProLong Gold to suppress z-axis spherical aberration; (3) use 0.17 mm thick coverslips for all measurements including CA measurement; (4) align imaging system and image using only a small region within the center of camera to suppress SD of CA; and (5) use warmed PFA fixation without pre-extraction or detergent in fixation solution.

Article Snippet: Cells were grown on acid washed #1.5 coverslips (~0.17 mm thick, Corning).

Techniques: Staining, Labeling, Imaging, Confocal Microscopy, Fluorescence, Standard Deviation, Extraction

(A) List of Delta, S(2D), S(3D), Z-offset, and zS(3D) (Z-offset subtracted) for the mean separation between CENP-T(M) and Hec1(9G3) in samples with or without large Z-offset. (B) List of Delta, S(2D), S(3D), Z-offset, and zS(3D) in samples with #1, #1.5, and #2 coverslips. The value zS(3D) was calculated from the S(3D) data after subtraction of the mean Z-offset in the middle of the spindle from the Sz values. All values were mean ±SD.

Journal: eLife

Article Title: An optimized method for 3D fluorescence co-localization applied to human kinetochore protein architecture

doi: 10.7554/eLife.32418

Figure Lengend Snippet: (A) List of Delta, S(2D), S(3D), Z-offset, and zS(3D) (Z-offset subtracted) for the mean separation between CENP-T(M) and Hec1(9G3) in samples with or without large Z-offset. (B) List of Delta, S(2D), S(3D), Z-offset, and zS(3D) in samples with #1, #1.5, and #2 coverslips. The value zS(3D) was calculated from the S(3D) data after subtraction of the mean Z-offset in the middle of the spindle from the Sz values. All values were mean ±SD.

Article Snippet: Cells were grown on acid washed #1.5 coverslips (~0.17 mm thick, Corning).

Techniques:

Measured variability of coverslip thickness within #1, #1.5, and #2 coverslips.

Journal: eLife

Article Title: An optimized method for 3D fluorescence co-localization applied to human kinetochore protein architecture

doi: 10.7554/eLife.32418

Figure Lengend Snippet: Measured variability of coverslip thickness within #1, #1.5, and #2 coverslips.

Article Snippet: Cells were grown on acid washed #1.5 coverslips (~0.17 mm thick, Corning).

Techniques: